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ImageStreamX MarkII Amnis 量化成像分析流式細胞儀介紹
將流式的統(tǒng)計學力量和熒光顯微鏡的形態(tài)學內涵深度結合,讓您突破“散點圖”的單一數據模式,在傳統(tǒng)流式熒光信號強度參數基礎上,為您提供超過百種量化成像參數用以進行不同細胞群體的分析。
● 下一代的“先鋒級”流式細胞儀,流式發(fā)展*具有里程碑意義的劃時代產品;
● 先鋒級硬件配置,多7根激光器,5MESF極限級熒光靈敏度,好級分辨率;
● 真正的多參數分析,提供每個細胞超過100種量化參數,獲得更全面、更深入的流式數據;
● 除了熒光強度單一參數以外,還提供準確的影像學判定,一次可實現(xiàn)數萬乃至數十萬細胞的高內涵數據分析結果;
● 眾多*應用,細胞間相互作用,蛋白定位,內吞,核轉位等等,將流式細胞技術從單一表型分析工具拉升到個體和群體功能性研究的綜合性平臺。
ImageStreamX MarkII Amnis 量化成像分析流式細胞儀應用
ImageStreamX MarkII參數
● 激光器:標配為488、642和785nm激光器,可拓展375、405、561、592、642nm激光器
● 檢測通道:標配為6個檢測通道,包括1個明場、1個暗場和至多5個熒光檢測通道;可升級為12個檢測通道,包括2個明場、1個暗場(側向角散射)和至多10個熒光檢測通
● 物鏡:標配為40倍,可升級為20/40/60倍物鏡組
● 熒光靈敏度:小于5MESF
● 上樣體積:20-20 μl
Amnis ImageStreamX MarkII introduction:
The ImageStreamX is Amnis' second generation imaging flow cytometer and the result of over 10 years of development. The raw power of the ImageStreamX for cell analysis is unmatched: it produces up to 12 high resolution images of each cell directly in flow, at rates exceeding 1,000 cells per second, and with the fluorescence sensitivity of the best conventional flow cytometers. These breakthrough capabilities allow you to quantitate cellular morphology and the intensity and location of fluorescent probes on, in, or between cells, even in rare sub-populations and highly heterogeneous samples.
Though ImageStream technology is years ahead of anything else, it has been thoroughly proven. Statistically robust and objective ImageStream data have been published in over 150 peer-reviewed articles to date. By combining the speed, sensitivity, and phenotyping abilities of conventional flow cytometry with the detailed imagery and functional insights of microscopy, think of how the ImageStreamX will advance your research.
With the ImageStreamX you can:
● Image cells directly in suspension with the resolution of a 60X microscope and the fluorescence sensitivity of the best flow cytometers.
● Analyze highly heterogeneous samples and rare cell sub-populations at speeds exceeding 1,000 cells per second.
● Perform phenotypic and functional studies at the same time using up to five lasers and 12 images per cell.
● Quantitate virtually anything you can see using the IDEAS® software package's numerous pre-defined fluorescence and morphologic parameters.
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